| Parameters | Input values |
|---|---|
| Folder name : | Example data |
| Sequence name : | Test sequence |
| Reference genome : | BSgenome.Hsapiens.UCSC.hg19 |
| Type of experiment : | Cloning-BSP |
| Group order : | group1, group2 |
| Date of analysis : | 2023-06-09 17:28 |
| Parameters | Data files content |
|---|---|
| Sequence name : | Test sequence |
| Collections : | collection1, collection2 |
| Groups : | group1, group2 |
| Clones : | 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 |
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection1_group1_clone01_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection1_group1_clone02_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection1_group1_clone03_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection1_group1_clone04_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection1_group1_clone05_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection1_group1_clone06_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection1_group1_clone07_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection1_group1_clone08_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection1_group1_clone09_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection1_group1_clone10_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection1_group2_clone01_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection1_group2_clone02_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection1_group2_clone03_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection1_group2_clone04_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection1_group2_clone05_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection1_group2_clone06_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection1_group2_clone07_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection1_group2_clone08_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection1_group2_clone09_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection1_group2_clone10_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection2_group1_clone01_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection2_group1_clone02_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection2_group1_clone03_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection2_group1_clone04_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection2_group1_clone05_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection2_group1_clone06_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection2_group1_clone07_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection2_group1_clone08_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection2_group1_clone09_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection2_group1_clone10_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection2_group2_clone01_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection2_group2_clone02_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection2_group2_clone03_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection2_group2_clone04_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection2_group2_clone05_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection2_group2_clone06_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection2_group2_clone07_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection2_group2_clone08_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection2_group2_clone09_methdata.csv
D:/Documents/These/ABSP/results/Example data/Test
sequence/individual_results_cloning/data/Test
sequence_collection2_group2_clone10_methdata.csv
For cloning expermients, methylation percentages calculated from sequencing results are converted to 0% or 100% methylation status.
A CpG site is considered unmethylated (0%) when methylation percentage is between 0% and 20%.| chr16:68770595-68770596 | chr16:68770613-68770614 | chr16:68770646-68770647 | chr16:68770665-68770666 | chr16:68770670-68770671 | chr16:68770717-68770718 | Mean | SD | |
|---|---|---|---|---|---|---|---|---|
| collection1 group1 clone 1 | 0 | 0 | 0 | 100 | NA | 100 | 40.00 | 54.77 |
| collection1 group1 clone 2 | 0 | 0 | 100 | 0 | 100 | 100 | 50.00 | 54.77 |
| collection1 group1 clone 3 | 0 | 100 | 0 | 100 | 0 | 0 | 33.33 | 51.64 |
| collection1 group1 clone 4 | 0 | 100 | 0 | 0 | 0 | 100 | 33.33 | 51.64 |
| collection1 group1 clone 5 | 0 | 0 | 0 | 100 | 100 | NA | 40.00 | 54.77 |
| collection1 group1 clone 6 | NA | 0 | 0 | 100 | 0 | 0 | 20.00 | 44.72 |
| collection1 group1 clone 7 | 0 | 100 | 0 | 0 | 0 | 0 | 16.67 | 40.82 |
| collection1 group1 clone 8 | 0 | 100 | 0 | 0 | NA | 0 | 20.00 | 44.72 |
| collection1 group1 clone 9 | 0 | 0 | 0 | 100 | 100 | 100 | 50.00 | 54.77 |
| collection1 group1 clone 10 | 100 | 0 | 0 | 0 | 0 | 0 | 16.67 | 40.82 |
| collection1 group2 clone 1 | 100 | 0 | 100 | 100 | 100 | 100 | 83.33 | 40.82 |
| collection1 group2 clone 2 | 100 | 0 | 100 | 100 | 100 | 100 | 83.33 | 40.82 |
| collection1 group2 clone 3 | 0 | 100 | 100 | 100 | 100 | 100 | 83.33 | 40.82 |
| collection1 group2 clone 4 | 100 | 100 | 0 | 100 | 100 | 100 | 83.33 | 40.82 |
| collection1 group2 clone 5 | 100 | 0 | 0 | 100 | 100 | 100 | 66.67 | 51.64 |
| collection1 group2 clone 6 | 100 | 0 | 100 | 100 | 100 | 100 | 83.33 | 40.82 |
| collection1 group2 clone 7 | 100 | 0 | 100 | 100 | 100 | 100 | 83.33 | 40.82 |
| collection1 group2 clone 8 | 0 | 0 | 100 | 100 | 0 | 100 | 50.00 | 54.77 |
| collection1 group2 clone 9 | 0 | 100 | 100 | 0 | 100 | 100 | 66.67 | 51.64 |
| collection1 group2 clone 10 | 0 | 100 | 100 | 0 | 100 | 100 | 66.67 | 51.64 |
| collection2 group1 clone 1 | 0 | 100 | 100 | 0 | NA | 100 | 60.00 | 54.77 |
| collection2 group1 clone 2 | 0 | 0 | 100 | 100 | 0 | 0 | 33.33 | 51.64 |
| collection2 group1 clone 3 | 0 | 100 | 100 | 0 | 100 | 100 | 66.67 | 51.64 |
| collection2 group1 clone 4 | NA | NA | NA | NA | NA | NA | NaN | NA |
| collection2 group1 clone 5 | 100 | 100 | 0 | 0 | 0 | 100 | 50.00 | 54.77 |
| collection2 group1 clone 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0.00 | 0.00 |
| collection2 group1 clone 7 | 0 | 0 | 100 | 100 | 0 | 100 | 50.00 | 54.77 |
| collection2 group1 clone 8 | 0 | 0 | 0 | 100 | NA | 100 | 40.00 | 54.77 |
| collection2 group1 clone 9 | 0 | 100 | 0 | 0 | 0 | 100 | 33.33 | 51.64 |
| collection2 group1 clone 10 | 0 | 0 | 100 | 0 | NA | 0 | 20.00 | 44.72 |
| collection2 group2 clone 1 | 0 | 100 | 100 | 100 | 100 | 100 | 83.33 | 40.82 |
| collection2 group2 clone 2 | 0 | 100 | 100 | 0 | 100 | 100 | 66.67 | 51.64 |
| collection2 group2 clone 3 | 100 | 100 | 100 | 100 | 100 | 0 | 83.33 | 40.82 |
| collection2 group2 clone 4 | 100 | 0 | 100 | 100 | 100 | 0 | 66.67 | 51.64 |
| collection2 group2 clone 5 | 100 | 100 | 100 | 100 | 100 | 100 | 100.00 | 0.00 |
| collection2 group2 clone 6 | 100 | 0 | 100 | 100 | 0 | 100 | 66.67 | 51.64 |
| collection2 group2 clone 7 | 100 | 100 | 0 | 100 | 100 | 100 | 83.33 | 40.82 |
| collection2 group2 clone 8 | 0 | 100 | NA | 0 | 0 | 100 | 40.00 | 54.77 |
| collection2 group2 clone 9 | 100 | 100 | 100 | 0 | 100 | 100 | 83.33 | 40.82 |
| collection2 group2 clone 10 | 0 | 0 | 100 | 0 | 100 | 100 | 50.00 | 54.77 |
| chr16:68770595-68770596 | chr16:68770613-68770614 | chr16:68770646-68770647 | chr16:68770665-68770666 | chr16:68770670-68770671 | chr16:68770717-68770718 | Mean | SD | |
|---|---|---|---|---|---|---|---|---|
| collection1 group1 | 11.11 | 40.00 | 10.00 | 50.00 | 37.50 | 44.44 | 32.17 | 17.28 |
| collection1 group2 | 60.00 | 40.00 | 80.00 | 80.00 | 90.00 | 100.00 | 75.00 | 21.68 |
| collection2 group1 | 11.11 | 44.44 | 55.56 | 33.33 | 16.67 | 66.67 | 37.96 | 21.78 |
| collection2 group2 | 60.00 | 70.00 | 88.89 | 60.00 | 80.00 | 80.00 | 73.15 | 11.81 |
| Collection | Group | N | Methylation mean | SD | SEM | Confidence interval of 95% (±) |
|---|---|---|---|---|---|---|
| collection1 | group1 | 10 | 32.00 | 13.07 | 4.13 | 9.35 |
| collection1 | group2 | 10 | 75.00 | 11.78 | 3.73 | 8.43 |
| collection2 | group1 | 9 | 39.26 | 20.60 | 6.87 | 15.84 |
| collection2 | group2 | 10 | 72.33 | 17.92 | 5.67 | 12.82 |
| CpG number | CpG coordinate | Collection | Group | N | Methylation mean | SD | SEM | Confidence interval of 95% (±) |
|---|---|---|---|---|---|---|---|---|
| 1 | chr16:68770595-68770596 | collection1 | group1 | 9 | 11.11 | 33.33 | 11.11 | 25.62 |
| 1 | chr16:68770595-68770596 | collection1 | group2 | 10 | 60.00 | 51.64 | 16.33 | 36.94 |
| 1 | chr16:68770595-68770596 | collection2 | group1 | 9 | 11.11 | 33.33 | 11.11 | 25.62 |
| 1 | chr16:68770595-68770596 | collection2 | group2 | 10 | 60.00 | 51.64 | 16.33 | 36.94 |
| 2 | chr16:68770613-68770614 | collection1 | group1 | 10 | 40.00 | 51.64 | 16.33 | 36.94 |
| 2 | chr16:68770613-68770614 | collection1 | group2 | 10 | 40.00 | 51.64 | 16.33 | 36.94 |
| 2 | chr16:68770613-68770614 | collection2 | group1 | 9 | 44.44 | 52.70 | 17.57 | 40.51 |
| 2 | chr16:68770613-68770614 | collection2 | group2 | 10 | 70.00 | 48.30 | 15.28 | 34.56 |
| 3 | chr16:68770646-68770647 | collection1 | group1 | 10 | 10.00 | 31.62 | 10.00 | 22.62 |
| 3 | chr16:68770646-68770647 | collection1 | group2 | 10 | 80.00 | 42.16 | 13.33 | 30.16 |
| 3 | chr16:68770646-68770647 | collection2 | group1 | 9 | 55.56 | 52.70 | 17.57 | 40.51 |
| 3 | chr16:68770646-68770647 | collection2 | group2 | 9 | 88.89 | 33.33 | 11.11 | 25.62 |
| 4 | chr16:68770665-68770666 | collection1 | group1 | 10 | 50.00 | 52.70 | 16.67 | 37.70 |
| 4 | chr16:68770665-68770666 | collection1 | group2 | 10 | 80.00 | 42.16 | 13.33 | 30.16 |
| 4 | chr16:68770665-68770666 | collection2 | group1 | 9 | 33.33 | 50.00 | 16.67 | 38.43 |
| 4 | chr16:68770665-68770666 | collection2 | group2 | 10 | 60.00 | 51.64 | 16.33 | 36.94 |
| 5 | chr16:68770670-68770671 | collection1 | group1 | 8 | 37.50 | 51.75 | 18.30 | 43.27 |
| 5 | chr16:68770670-68770671 | collection1 | group2 | 10 | 90.00 | 31.62 | 10.00 | 22.62 |
| 5 | chr16:68770670-68770671 | collection2 | group1 | 6 | 16.67 | 40.82 | 16.67 | 42.84 |
| 5 | chr16:68770670-68770671 | collection2 | group2 | 10 | 80.00 | 42.16 | 13.33 | 30.16 |
| 6 | chr16:68770717-68770718 | collection1 | group1 | 9 | 44.44 | 52.70 | 17.57 | 40.51 |
| 6 | chr16:68770717-68770718 | collection1 | group2 | 10 | 100.00 | 0.00 | 0.00 | 0.00 |
| 6 | chr16:68770717-68770718 | collection2 | group1 | 9 | 66.67 | 50.00 | 16.67 | 38.43 |
| 6 | chr16:68770717-68770718 | collection2 | group2 | 10 | 80.00 | 42.16 | 13.33 | 30.16 |
Student’s T test (two-samples, unpaired).
| Collection | Compared group 1 | Compared group 2 | N group 1 | N group 2 | p-value | p-value significance level |
|---|---|---|---|---|---|---|
| collection1 | group1 | group2 | 10 | 10 | 4.30e-07 | **** |
| collection2 | group1 | group2 | 9 | 10 | 1.88e-03 | ** |
Student’s T test (two-samples, unpaired).
| CpG number | CpG coordinate | Collection | Compared group 1 | Compared group 2 | N group 1 | N group 2 | p-value | p-value significance level |
|---|---|---|---|---|---|---|---|---|
| 1 | chr16:68770595-68770596 | collection1 | group1 | group2 | 9 | 10 | 0.025300 | * |
| 2 | chr16:68770613-68770614 | collection1 | group1 | group2 | 10 | 10 | 1.000000 | ns |
| 3 | chr16:68770646-68770647 | collection1 | group1 | group2 | 10 | 10 | 0.000624 | *** |
| 4 | chr16:68770665-68770666 | collection1 | group1 | group2 | 10 | 10 | 0.178000 | ns |
| 5 | chr16:68770670-68770671 | collection1 | group1 | group2 | 8 | 10 | 0.028500 | * |
| 6 | chr16:68770717-68770718 | collection1 | group1 | group2 | 9 | 10 | 0.013300 | * |
| 1 | chr16:68770595-68770596 | collection2 | group1 | group2 | 9 | 10 | 0.025300 | * |
| 2 | chr16:68770613-68770614 | collection2 | group1 | group2 | 9 | 10 | 0.288000 | ns |
| 3 | chr16:68770646-68770647 | collection2 | group1 | group2 | 9 | 9 | 0.132000 | ns |
| 4 | chr16:68770665-68770666 | collection2 | group1 | group2 | 9 | 10 | 0.269000 | ns |
| 5 | chr16:68770670-68770671 | collection2 | group1 | group2 | 6 | 10 | 0.012900 | * |
| 6 | chr16:68770717-68770718 | collection2 | group1 | group2 | 9 | 10 | 0.541000 | ns |
Boxplots represent methylation median and quartiles of each group with errors bars. Methylation mean are represented as diamond shapes.
The p-values and p-value significance levels are determined by Student's T tests (two-samples, unpaired).
Individual clone methylation is represented as a round shape and the methylation mean of clones as a diamond shape.
The p-values and p-value significance levels are determined by Student's T tests (two-samples, unpaired).
Each points represent the methylation mean of clones or replicates
for a given CpG site.
The p-values and p-value significance levels are determined by
Kruskal-Wallis tests.
Test sequence_boxplot_collection1_means_no_p.png
Test sequence_boxplot_collection1_means_psign.png
Test sequence_boxplot_collection1_means_pval.png
Test sequence_boxplot_collection1_positions_no_p.png
Test sequence_boxplot_collection1_positions_psign.png
Test sequence_boxplot_collection1_positions_pval.png
Test sequence_boxplot_collection2_means_no_p.png
Test sequence_boxplot_collection2_means_psign.png
Test sequence_boxplot_collection2_means_pval.png
Test sequence_boxplot_collection2_positions_no_p.png
Test sequence_dendro_clones_collection1_group1.png
Test sequence_dendro_clones_collection1_group2.png
Test sequence_dendro_clones_collection2_group1.png
Test sequence_genomic_clones_as-is_collection1_group1.png
Test sequence_genomic_clones_as-is_collection1_group2.png
Test sequence_genomic_clones_as-is_collection2_group1.png
Test sequence_genomic_clones_as-is_collection2_group2.png
Test sequence_genomic_clones_by-meth_collection1_group1.png
Test sequence_genomic_clones_by-meth_collection1_group2.png
Test sequence_genomic_clones_by-meth_collection2_group1.png
Test sequence_genomic_clones_by-meth_collection2_group2.png
Test sequence_genomic_clones_clust_collection1_group1.png
Test sequence_genomic_clones_clust_collection1_group2.png
Test sequence_genomic_clones_clust_collection2_group1.png
Test sequence_genomic_clones_clust_collection2_group2.png
Test sequence_genomic_groups_as-is.png
Test sequence_genomic_groups_by-group.png
Test sequence_lollipop_clones_as-is_condensed_collection1_group1.png
Test sequence_lollipop_clones_as-is_condensed_collection1_group2.png
Test sequence_lollipop_clones_as-is_condensed_collection2_group1.png
Test sequence_lollipop_clones_as-is_condensed_collection2_group2.png
Test sequence_lollipop_clones_as-is_proportional_collection1_group1.png
Test sequence_lollipop_clones_as-is_proportional_collection1_group2.png
Test sequence_lollipop_clones_as-is_proportional_collection2_group1.png
Test sequence_lollipop_clones_as-is_proportional_collection2_group2.png
Test sequence_lollipop_clones_by-meth_condensed_collection1_group1.png
Test sequence_lollipop_clones_by-meth_condensed_collection1_group2.png
Test sequence_lollipop_clones_by-meth_condensed_collection2_group1.png
Test sequence_lollipop_clones_by-meth_condensed_collection2_group2.png
Test sequence_lollipop_clones_by-meth_proportional_collection1_group1.png
Test sequence_lollipop_clones_by-meth_proportional_collection1_group2.png
Test sequence_lollipop_clones_by-meth_proportional_collection2_group1.png
Test sequence_lollipop_clones_by-meth_proportional_collection2_group2.png
Test sequence_lollipop_clones_clust_condensed_collection1_group1.png
Test sequence_lollipop_clones_clust_condensed_collection1_group2.png
Test sequence_lollipop_clones_clust_condensed_collection2_group1.png
Test sequence_lollipop_clones_clust_condensed_collection2_group2.png
Test sequence_lollipop_clones_clust_proportional_collection1_group1.png
Test sequence_lollipop_clones_clust_proportional_collection1_group2.png
Test sequence_lollipop_clones_clust_proportional_collection2_group1.png
Test sequence_lollipop_clones_clust_proportional_collection2_group2.png
Test sequence_lollipop_groups_as-is_condensed.png
Test sequence_lollipop_groups_as-is_proportional.png
Test sequence_lollipop_groups_by-group_condensed.png
Test sequence_lollipop_groups_by-group_proportional.png
Test sequence_lollipop_groups_by-meth_condensed.png
Test sequence_lollipop_groups_by-meth_proportional.png
Test sequence_meth_profile_collection1_condensed_no_p.png
Test sequence_meth_profile_collection1_condensed_psign.png
Test sequence_meth_profile_collection1_condensed_pval.png
Test sequence_meth_profile_collection1_proportional_no_p.png
Test sequence_meth_profile_collection1_proportional_psign.png
Test sequence_meth_profile_collection1_proportional_pval.png
Test sequence_meth_profile_collection2_condensed_no_p.png
Test sequence_meth_profile_collection2_condensed_psign.png
Test sequence_meth_profile_collection2_condensed_pval.png
Test sequence_meth_profile_collection2_proportional_no_p.png
Test sequence_meth_profile_collection2_proportional_psign.png
Test sequence_meth_profile_collection2_proportional_pval.png
Test sequence_descriptive_statistics_means.csv
Test sequence_descriptive_statistics_means.xlsx
Test sequence_descriptive_statistics_positions.csv
Test sequence_descriptive_statistics_positions.xlsx
Test sequence_methylation_table_clones.csv
Test sequence_methylation_table_clones.xlsx
Test sequence_methylation_table_groups.csv
Test sequence_methylation_table_groups.xlsx
Test sequence_t_test_table_means.csv
Test sequence_t_test_table_means.xlsx